• Sugandha Angerish

Chromatography is a vital biophysical method for qualitative and quantitative analysis that allows for the identification, separation as well as purification of the components of a mixture. Size or shape, total charge, hydrophobic groups on the surface, and binding ability with the stationary phase are all factors that may be used to purify proteins. Ion exchange, surface adsorption, partition, or size exclusion are four separation strategies based on molecule properties and interaction type. Other chromatography techniques, such as column, thin layer, and paper chromatography, are based on the stationary bed. One of the most popular methods of protein purification is column chromatography. The primary goal of this article is to get a better understanding of chromatography techniques, kinds, and applications. Chromatographic methods will give increasingly precise findings in the future. Affinity chromatography is increasingly being used to explore PTM protein and protein interactions, particularly with the objective of creating innovative universal tag systems and chemical derivatization methods for peptide affinity selection.

Affinity Chromatography, Column Chromatography, Gas Chromatography, Mobile Phase, Stationary Phase.

"Study of Chromatography Techniques and Its Application", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.5, Issue 9, page no.406-411, September 2018, Available :http://www.jetir.org/papers/JETIRFH06067.pdf

"Study of Chromatography Techniques and Its Application", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.5, Issue 9, page no. pp406-411, September 2018, Available at : http://www.jetir.org/papers/JETIRFH06067.pdf