• Dr Ranjini P
• Dr. Shailasree S
• Dr. Ramachandra Kini K

Abstract: Plant species harbor multiple, diverse, disease resistance (R) genes to a wide range of pathogens. Downy mildew caused by Sclerospora graminicola (Sacc.) shroet infection is a ruinous disease of pearl millet [Pennisetum glaucum (L.) R. Br.]. In our earlier studies using candidate gene approach, 22 resistance gene analogues (RGAs) were identified. Of these, a clone RGPM213 was shown to encode resistant protein having serine-threonine kinase domain and its transcript was upregulated following S. graminicola infection, β-amino butyric acid and chitosan inducer treatments. In the present study, RACE-PCR was employed to isolate RGPM 213 gene sequence. The complete cDNA contained 3616 bp, with one open reading frame encoding 778 amino acids. The sequence was deposited in the GenBank (HQ214676). The 5` RACE-PCR sequence showed 99% identity to partial RGPM 213 sequence. BLASTX analysis showed that it shared more than 75% identity to various R proteins containing NBS-LRR region from Oryza sativa and Saccharum officinarum. The Conserved domain analysis showed the encoded protein belonged to NTPases superfamily. 5` RACE-PCR of RGPM 213 product showed ORF of 778 aminoacid residues from 278bp to 2616bp after ORF finder analysis. SMART database searches revealed presence of CC domain followed by NBS domain and LRR domain. The 3` RACE-PCR showed a UTR region and poly A tail. Protein interaction network and pathway analysis of HQ214676 was established and it predicted eliciting responses of biotic stress against pathogen in pearl millet. Modeling and docking RGPM 213 NBS-LRR like protein with PIF3 protein predicted binding residues for kinases.

Resistance gene analog; CC-NBS-LRR; protein interaction networks; HQ214676 analysis.

"Serine Threonine Protein Kinase – RGPM 213 gene isolated from pearl millet by RACE-PCR", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.10, Issue 1, page no.c222-c249, January-2023, Available :http://www.jetir.org/papers/JETIR2301228.pdf

"Serine Threonine Protein Kinase – RGPM 213 gene isolated from pearl millet by RACE-PCR", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.10, Issue 1, page no. ppc222-c249, January-2023, Available at : http://www.jetir.org/papers/JETIR2301228.pdf