• Dr.M.Chandran

Indigofera tinctoria is a best medicine to remove all toxicity in human body. The illiterate people in rural India, (even an educated) believe the Hindu sorceress to kill their enemy by giving some poisonous substance secretly through food. These poisonous substances may be plant origin or the composition made by heavy metals. Suppose if any person affected by these types of poison unknowingly. They were treated by Indigofera tinctoria leaf powder to remove all poison from the body by some traditional healer. Hence, in the present investigation, the FTIR analyses were done to evaluate the existence of bioactive phytocompounds in methanol leaf extract of plant Indigofera tinctoria. The methanol extract was taken to column chromatography to separate the various fractions present in extract. The obtained results showed two major fractions. They are, fraction –I and II. These fractions were analyzed in FTIR.The obtained results showed that the fraction I of Indigofera tinctoria shows the following peak values such as 420.48, 470.63, 530.42, 597.93, 669.3, 754.17, 900.76, 1076.28, 1234.44, 1382.96, 1514.12, 1612.49, 1705.07, 1890.24, 1965.46 ,2119.77, 2208.49, 2362.8, 2929.87, 3392.79, 3774.69, 3878.85, 3927.07 and 3975.29 indicates the existence of bands such as C-I Strech,C- O-H Bending, CH out-of-plane-deformation,SO2 sym stretch, Skeletal vibration, SO2 antisym stretch,CH3 antisym deformation,COO – antisym stretch, C═O stretch, P- H stretch, CH antisym and sym stretching, CH antisym and sym stretching, OH stretch, OH stretch and OH stretch and the presence of bands related functional groups such as Iodo Compounds,C- OH in alcohol,o-disubst benzene,SO3H in sulfonic acids,t-butyl in hydrocarbon,SO2 in sulfonyl chloride,CH3 Aliphatic compounds,COO – Carboxylic acid salts,C=O in carboxylic acids,-PH in phosphines,-CH3and-CH2 in aliphatic compounds,-CH3and-CH2 in aliphatic compounds,-OH in alcohol and phenols,-OH in alcohol and phenols and -OH in alcohol and phenols respectively. The fraction II of Indigofera tinctoria shows the following peak values such as 418.55, 466.77, 601.79, 1114.86, 1354.03, 1427.32, 1620.21, 2931.8, 3404.36 and 3533.59 indicates the existence of bands such as Cl-C═O plane deformation,out of plane ring bending,SO2 scissoring,C-N Stretch,NO2 sym stretch,OH Plane bending,C=C stretch,CH- antisym and sym stretching, OH stretch (Solids and Liquids) and OH stretch (dil soln) and the presence of bands related functional groups such as Cl-C═O in acid chlorides ,Napthalenes,SO2 in sulfones,C-NH2 in primary aliphatic amines,NO2 in aromatic nitro compounds, Carboxylic acids, C=C in vinyl ethers,CH3 and –CH2,-OH in alcohols and phenols, and -OH in alcohols and phenols respectively.

FTIR, Indigofera tinctoria, Neeli, Fabaceae

"FT-IR analysis of methanolic extract of leaf of Indigofera tinctoria", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.1, Issue 5, page no.408-414, October-2014, Available :http://www.jetir.org/papers/JETIR1701463.pdf

"FT-IR analysis of methanolic extract of leaf of Indigofera tinctoria", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.1, Issue 5, page no. pp408-414, October-2014, Available at : http://www.jetir.org/papers/JETIR1701463.pdf