• Upasana Deb

Nature has a unique way of degrading the unwanted wastes which are a burden to the society. For this, nature involves the microorganisms, which degrade the waste for their food and in the process gives out metabolites like enzymes such as cellulases, xylanasses, mannoses etc. and antibiotics. So, it becomes a necessity and very interesting to study those microbes for a better developed and cleaner society. Hence, a study has been done on degraded bamboo dust from Nagaon Paper Mill Guwahati, Assam, which is an unexplored area regarding microbial diversity especially on bamboo waste. Six bacteria were isolated from the sample by serial dilution and spread-plate technique. All the strains were gram-positive and spore-forming. With the help of biochemical characterisation, the exoenzyme and endoenzyme activities of the cells were observed. The exo-enzyme activities were studied by tests like hydrolysis of starch, lipid, casein, gelatine etc. Endoenzyme activities were understood by tests like carbohydrate fermentation, H2S production, catalase, urease, oxidase, IMViC test, nitrate reduction etc. The results of biochemical tests were put in an online tool, the ABIS online tool, accessed through www. tgw.1916.net. Bacterial characterisation and identification depicted that all the isolates belonged to the Genus Bacillus. Identification of the strains was further validated by using 16 s rRNA sequencing.

Biochemical characterisation, molecular characterisation, bacterial isolates, bamboo dust

"Biochemical and molecular characterisation bacterial Isolates from the waste products of Nagaon Paper Mill", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 5, page no.501-508, May-2019, Available :http://www.jetir.org/papers/JETIR1905274.pdf

"Biochemical and molecular characterisation bacterial Isolates from the waste products of Nagaon Paper Mill", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 5, page no. pp501-508, May-2019, Available at : http://www.jetir.org/papers/JETIR1905274.pdf