• Sonu A.Varma
• Neha Tiwari
• Pragnesh Patani

Sitagliptin is a dipeptidyl-peptidase inhibitor (DPP-4 inhibitor) Synonyms of sitagliptn is sitagliptina that has recently been approved for the therapy of type 2 diabetes. Like other DPP-4 inhibitors its action is mediated by increasing levels of the incretin hormones glucagon-like peptide-1 (GLP-1) and gastric inhibitory polypeptide. Sitagliptin is effective in lowering HbA1c, and fasting as well as postprandial glucose in monotherapy and in combination with other oral antidiabetic agents. It stimulates insulin secretion when hyperglycemia is present and inhibits glucagon secretion. Absorption of Sitagliptin is 87% orally bioavailable and taking it with or without food does not affect its pharmacokinetics4. Sitagliptin reaches maximum plasma concentration in 2 hours. Route of elimination Approximately 79% of sitagliptin is excreted in the urine as the unchanged parent compound. 87% of the dose is eliminated in the urine and 13% in the feces .A new simple and precise reverse phase high performance liquid chromatographic method has been developed and subsequently validated for the estimation of Sitagliptin phosphate monohydrate in bulk and its pharmaceutical dosage form. The assay of Sitagliptin was found to be 99.89 %. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise, reliable, accurate and economical which is useful for the routine determination of Sitagliptin phosphate in bulk and its pharmaceutical dosage form. This assessment encompasses various analytical methods such as spectrometry and spectrophotometric , ultra performance liquid chromatography (UPLC), high performance thin layer chromatography (HPTLC) and gas chromatography-mass spectrometry (GC-MS) for the estimation of sitagliptin in single and/or in combination.

Sitagliptin Phosphate, Analytical methods, Type-2 d,iabetes, method validation

"Analytical Methods For Estimation Of Sitagliptin For Bulk Tablet And Dosage Form", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 5, page no.1173-1183, May-2019, Available :http://www.jetir.org/papers/JETIR1905S76.pdf

"Analytical Methods For Estimation Of Sitagliptin For Bulk Tablet And Dosage Form", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 5, page no. pp1173-1183, May-2019, Available at : http://www.jetir.org/papers/JETIR1905S76.pdf