• Dr. Narpat Singh Deora

Various experiments viz. types of media (MS basal, MS three- fourth, MS half, B5 and WP) and growth regulators (auxin, cytokinin) with various concentration were designed in order to study the bud breaking or multiple shoot induction. Out of various media and growth regulators tested the maximum number of explants were responded on MS full strength medium supplemented with NAA 0.1 mg/l + BAP 5.0 mg/l +additives. Different type of cytokinin (2-IP, KN, BAP) with concentration from 0.25-2.5 mg/l) were tested but best result from subcultured shoot were found on MS medium supplemented with IAA 0.1 mg/l + BAP 2.5 mg/l+ additives (ascorbic acid 50 mg/l, citric acid 25 mg/l L-arginine 25 mg/l and adenine sulphate 25 mg/l).These culture were incubated at temperature ranging from 25C to 35C under different regime of light for shoot induction.

in vitro, medium, subculture, inoculation, explant

"In vitro studies on Capparis decidus. A multipurpose plant of arid region.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.7, Issue 11, page no.11-13, November-2020, Available :http://www.jetir.org/papers/JETIR2011431.pdf

"In vitro studies on Capparis decidus. A multipurpose plant of arid region.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.7, Issue 11, page no. pp11-13, November-2020, Available at : http://www.jetir.org/papers/JETIR2011431.pdf