• Akshay paliwal
• Dr. Neha Tiwari
• Dr. Pragnesh patani

A simple, rapid, economical, precise and accurate RP-HPLC method simultaneous estimation of Chlorthalidone, metoprolol succinate and Telmisartan in tablets has been developed. A reverse phase high performance liquid chromatographic method was developed for the simultaneous estimation of Chlorthalidone, Metoprolol succinate and Telmisartan in tablets has been developed. The separation was achieved by Hypersil BDS C18 (250mm x 4.6mm, 5μm) column and Buffer (0.5M potassium dihydrogen ortho phosphate, pH was adjusted with 1% orthophosphoric acid, pH3.0): methanol (50:50v/v) as a mobile phase at a flow rate of 1ml/min. detection was carried out at 215nm Retention time of Chlorthalidone- 6.690, Metoprolol – 8.813 and Telmisartan- 4.887minute. The method has been validated for linearity, accuracy and precision. Linearity observed for Chlorthalidone 3.12- 18.75 μg/ml, Metoprolol succinate 12.5- 75 μg/ml and Telmisartan 10-60μg/ml. Developed method was found to be accurate, precise and rapid for simultaneous estimation of Chlorthalidone, metoprolol succinate and Telmisartan in Tablets.

KEYWORDS: Chlorthalidone, Metoprolol succinate, Telmisartan, RP-HPLC method, Validation.

"RP-HPLC METHOD DEVELOPMENT & VALIDATION FOR SIMULTANEOUS ESTIMATION OF CHLORTHALIDONE, METOPROLOL SUCCINATE AND TELMISARTAN IN TABLETS", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.8, Issue 5, page no.f804-f811, May-2021, Available :http://www.jetir.org/papers/JETIR2105773.pdf

"RP-HPLC METHOD DEVELOPMENT & VALIDATION FOR SIMULTANEOUS ESTIMATION OF CHLORTHALIDONE, METOPROLOL SUCCINATE AND TELMISARTAN IN TABLETS", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.8, Issue 5, page no. ppf804-f811, May-2021, Available at : http://www.jetir.org/papers/JETIR2105773.pdf