• Sanjay B. Sonawale
• Dnyaneshvar J. Katkar

Iron (III) serves as a key component of the enzyme systems and proteins with vital functions at optimum levels. Its excessive intake causes siderosis while its deficiency causes anemia therefore, it is of interest to determine iron (III) in biological samples, foodstuffs and pharmaceutical formulations. A rapid sensitive and selective spectrophotometric method for the determination of trace amount of iron(III) has been developed based on the extraction of iron(III) from an aqueous solution of pH 1.9-2.5 with bis(2,4,4-trimethyl pentyl dithiophosphonic acid in toluene. The optimum shaking time and reagent concentration for the maximum absorption have been evaluated. The green-colored toluene extract exhibits maximum absorption at 613nm the molar absorptivity was found to be 5.048x103/mol-1cm- 1. Beers law is obeyed in the range of 0.2-4.8 ppm of iron (iii). sandell's sensitivity is 0.102µg iron (III) cm. 2. The log-log plot at pH 2.2 gave a linear graph with a slope of 2.8 indicating the complex extracted into toluene is 1:3 complex of metal reagent. The effect of various diverse cations and the anions on the extraction of 50µg of iron (III) have been studied and found that method is selected. The method has been applied for the determination of iron (III) in the human hair sample, some pharmaceutical formulations and foodstuffs and compared with the ICP-AES method.

Iron (III), bis-(2-4, 4-trimethyl pentyl dithiophosphonic acid, spectrophotometry.

"Spectrophotometric determination of Iron (III) in biological samples", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.9, Issue 2, page no.b429-b432, February-2022, Available :http://www.jetir.org/papers/JETIR2202148.pdf

"Spectrophotometric determination of Iron (III) in biological samples", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.9, Issue 2, page no. ppb429-b432, February-2022, Available at : http://www.jetir.org/papers/JETIR2202148.pdf