Abstract
Six streptomyces sp ESS-4 (Streptomyces fradiae), DVS-11 (Streptomyces olivoviridis), KZSS-6 (Streptomyces koyangensis), NVS-10 (Streptomyces flavofuscus), TSS-3 (Streptomyces griseorubens), and TVS-2 (Streptomyces lunaelactis) were previously reported to antagonistic activity in fungal pathogens, Plant Growth Promoting traits, Greenhouse and field conditions in black gram. These isolates were characterized for their antibacterial activity for streak plate method, antibiotic resistances, and optimization of pH, salinity, temperature, carbon sources, and heavy metals. Theses streptomyces sp produce halo-zone range between 14 to 19 cm, for the pH grow range from 3 to 13, salinity 0% to 12%, temperature 20oC to 50oC, four carbon sources are used for carbon utilization such as glucose, fructose, sucrose, and starch, and heavy metals tolerances. Out of six streptomyces species KZSS-6 (Streptomyces koyangensis) was better results, and more efficient. So KZSS-6 strain was further evaluated on the greenhouse and black gram field conditions. The KZSS-6 strain was found to enhance plant root length, and yield. In greenhouse condition the plant was grown in height 8 to 14cm, root length1 to 6cm, seeds 2 to 5 cm, total plant weight is 0.20 to 0.60mg, and total dry weight for plant 7 to 14mg. In the field experiment plant was grown in the root height and weight (10 to 13cm, 0.25 to 0.50mg), shoot length and weight (3.10 to 3.50cm), stem height and stem weight (22 to 32 cm, 4.0 to 4.25 gm), plant weight (5.50 to 7.50 gm), number of fruits weight (10, 100, and 1000) (0.4 to 0.50, 2.50 to 5.0 and 22 to 45 gm), and finally the yield (11.5 to 29.50 tons).