• R Meenakshi
• R Shyam sunder

It has been developed and validated an accurate, sensitive, precise, quick, and isocratic reverse phase HPLC (RP-HPLC) technique for combined quantification of Etodolac, and Dexamethasone in raw materials and pharmaceutical combined dispensing kit contains tablet dosage forms. With acetonitrile as the organic solvent, the best separation was achieved on a 250 mmx4.6 mm i.d., 5µ-particle size Inertsil®-Octadecyl-silyl-3V-Reverse-Phase-C18-column with acetonitrile as the non-polar-modifier and ortho-phosphoric acid (0.1%v/v) in water in the isocratic mode of elution as mobile phase solvent at a speed of 1.0 mL.min−1. UV detection was at 245-nm. Etodolac's was 2.0 minutes, and Dexamethasone's was 2.9 minutes. With a correlation coefficient of about 0.9999, peak-response was obtained as function of concentration over the range of 1.6-9.6 µg/mL for dexamethasone and 60-360 µg/mL for etodolac. Etodolac, and Dexamethasone were shown to have a percentage assay of 99.57, and 99.97, respectively. Etodolac, and Dexamethasone each have a limit of detection of 0.2 mcg/mL and 0.0016 mcg/mL, respectively. Etodolac, and Dexamethasone each have a limit of quantification (LOQ) of 0.6 mcg/mL and 0.0048 mcg/mL, respectively. The presence of excipients in the formulation had no effect on the assay method. The procedure is appropriate for use in QC- laboratories since it is quick and precise.

Etodolac, Dexamethasone, Isocratic-RP-HPLC, and Fixed dosage forms

"ISOCRATIC RP-LC METHOD FOR COMBINED QUANTIFICATION OF THE ETODOLAC AND DEXAMETHASONE IN COMBINATION TABLETS ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.10, Issue 6, page no.j579-j585, June-2023, Available :http://www.jetir.org/papers/JETIR2306967.pdf

"ISOCRATIC RP-LC METHOD FOR COMBINED QUANTIFICATION OF THE ETODOLAC AND DEXAMETHASONE IN COMBINATION TABLETS ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.10, Issue 6, page no. ppj579-j585, June-2023, Available at : http://www.jetir.org/papers/JETIR2306967.pdf