• PRAVIN V GADAKH
• DEVENDRA V DESHMUKH

Biosurfactants are amphiphilic compounds having an unique properties that allows their use and which are natural replacement for chemically synthesized surfactant. Micro-organisms produced these biosurfactants on their surface or may also secret extracellularly and it contains both hydrophilic and hydrophobic moieties which reduces the surface and interfacial tension of the surface and interface respectively. In the present study a screening was done to check the ability of microorganisms to produce biosurfactant from various petroleum sites. Out of 40 soil samples 20 bacterial species were isolated. Based on morphology and biochemical characterization the bacterial species were identified and only 9 species were having the ability to produce bio surfactants. These bacterial species were also screened for their ability to produce different hydrolytic enzymes like amylase, lipase and proteases using standard methods. This study has opened new avenues for biosurfactant studies and their application in bioremediation of contaminated sites as well as in industrial purpose. Further work on purification and large scale production using cheap raw substrate is needed to explore these bacteria.

Biosurfactants, hydrocarbons, surface tension, functional properties

"SCREENING OF BIO SURFACTANT PRODUCING BACTERIA AND THEIR ABILITY TO PRODUCE INDUSTRIAL IMPORTANT ENZYMES", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.10, Issue 7, page no.h349-h354, July-2023, Available :http://www.jetir.org/papers/JETIR2307744.pdf

"SCREENING OF BIO SURFACTANT PRODUCING BACTERIA AND THEIR ABILITY TO PRODUCE INDUSTRIAL IMPORTANT ENZYMES", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.10, Issue 7, page no. pph349-h354, July-2023, Available at : http://www.jetir.org/papers/JETIR2307744.pdf