• Dr.Roselin Polugari
• N.Vaishnavi Goud

Nanoparticles can be synthesized by using microbes and by plant extracts. Under the favourable conditions, size and surface of nanoparticles can be easily altered to achieve both passive and active transportation of drug Nanotechnology is recognized as the study of particle with a minimum of one dimension in nanometers. Synthesis of nanoparticles can be done through three methods like physical, chemical, and green synthesis methods. Green synthesis can be done using microorganisms, enzymes, or plant extracts. This project work is based and focused on the green synthesis method due to its non-toxic and eco-friendly process. In this project, Zinc (II) ion solution is reduced into ZnO nanoparticles using plants like Hibiscus subdariffa, Ficus religiosa and Catharanthus roseus. The significant antibacterial activity of ZnO NPs was observed against Staphylococcus aureus MTCC 9760 (S. aureus), Streptococcus pyogenes MTCC 1926 (S. pyogenes), Bacillus cereus MTCC 430 (B. cereus), Pseudomonas aeruginosa MTCC 424 (P. aeruginosa), Proteus mirabilis MTCC 3310 (P. mirabilis) and Escherichia coli MTCC 40 (E. coli). The synthesized ZnO NPs have shown antibacterial efficacy against both Gram-positive and Gram-negative pathogens. Overall, the results elucidated a rapid, costeffective, environmentally friendly and convenient method for ZnO NPs synthesis, which could be used as a potential antimicrobial agent against drug resistant microbes. Minimum inhibitory concentrations (MICs) test is performed to determine the susceptibility of organisms to the nanoparticles.

ZnO nanoparticles, Antimicrobial activity

"“ANTIMICROBIAL ACTIVITY OF GREEN SYNTHESISED ZINC OXIDE NANOPARTICLE USING HIBISCUS SUBDARIFFA, FICUS RELIGIOSA AND CATHARANTHUS ROSEUS”", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.10, Issue 11, page no.f140-f149, November-2023, Available :http://www.jetir.org/papers/JETIR2311515.pdf

"“ANTIMICROBIAL ACTIVITY OF GREEN SYNTHESISED ZINC OXIDE NANOPARTICLE USING HIBISCUS SUBDARIFFA, FICUS RELIGIOSA AND CATHARANTHUS ROSEUS”", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.10, Issue 11, page no. ppf140-f149, November-2023, Available at : http://www.jetir.org/papers/JETIR2311515.pdf