• Dr. S. VINODHINI
• RANJITH K
• KISHORE G
• KALAIVANI M

Natural dyes are colored substances found in various plants, including fruits, stems, leaves, bark, and roots. Commercially, these biodegradable dyes were used to color textiles, pharmaceuticals, cosmetics, leather, and paint. Natural dyes are extremely beneficial as alternatives to artificially manufactured dyes. The current work analyzes the extraction of plant dyes from fresh and dry leaves of Lawsonia inermis and Eclipta prostrata using ethanol and water, as well as their efficiency as a stain for angiospermic stem tissues. A 10% w/v crude extract of Lawsonia inermis and Eclipta prostrata fresh and dried leaves in ethanol and water was used to stain the stem cross section of angiospermic plants such as Boerhavia chinensis L. (Dicot) and Stenotaphrum secundatum Kuntze (Monocot). Dye extracted from Lawsonia inermis and Eclipta prostrata fresh and dry leaves in ethanol profusely stained the vascular bundle of the angiospermic stem cross section of Boerhavia chinensis, but ground parenchyma was only slightly stained. Dye extracted from Lawsonia inermis and Eclipta prostrata leaves in 10% w/v ethanol through stained the sclerenchyma of Stenotaphrum secundatum Kuntze stem cross section more effectively than parenchyma in Boerhavia chinensis. Xylem cells were highly stained within each vascular bundle, but the cortex and medulla were less so. This opens up a new channel of research and further inquiries to uncover better methods of extracting the dye in concentrated form in more diverse solvents and better methods of creating stains.

Natural plants dyes, Mordants, Stem Tissues, plant anatomy.

"STAINING EFFECT OF DYE EXTRACTED FROM LEAVES OF Lawsonia inermis L., & Eclipta prostrata L., ON ANGIOSPERMIC STEM TISSUES ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.11, Issue 4, page no.j721-j734, April-2024, Available :http://www.jetir.org/papers/JETIR2404989.pdf

"STAINING EFFECT OF DYE EXTRACTED FROM LEAVES OF Lawsonia inermis L., & Eclipta prostrata L., ON ANGIOSPERMIC STEM TISSUES ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.11, Issue 4, page no. ppj721-j734, April-2024, Available at : http://www.jetir.org/papers/JETIR2404989.pdf