• Danladi W,D.
• Ishaleku, D.
• Ngwai, Y.B.
• Okunlola O.I.
• Igbawua, I.N.

“Suya” product contaminated with Escherichia coli (E. coli) continue to be a food safety concern, economic losses and has the potential to dissemination infectious diseases and antimicrobial resistance.. This study was carried out to determine the antimicrobial resistance profile and phylogrouping of E. coli isolated from “Suya” sold in Nasarawa State, Nigeria. A total of two hundred and ten (210) “Suya” samples were collected and screened and E. coli was isolated and identified using standard microbiological techniques. The antibiotic susceptibility test was carried out using Kirby-Bauer disc diffusion method on Mueller-Hinton agar against ten antibiotics. Isolates were confirmed as ESBL producers phenotypically by Double Disc Synergy Test (DDST) while ESBL genes and phylogenetic grouping were determined by Polymerase Chain Reaction (PCR). Out of 210 samples collected, 70(33.3%) E. coli were isolated. Isolates were highly resistant to amoxicillin/clavulanic acid 7(77.7%) in Keffi and 6(75.0%) in Nasarawa of Nasarawa West Senatorial District. These isolates were also resistant to cefoxitin, streptomycin, and co-trimoxazole 6(75.0%) in Nasarawa, Karu and Nasarawa Eggon however, none of the isolates were resistance to gentamicin 0(0.0%) at Lafia in Nasarawa South Senatorial District. Most of the E. coli isolates had multiple resistance (MAR) to at least five antibiotics (MAR Index of = 0.5) and the most frequent MAR Index was 0.8 with 25.0% occurrence and all were Multi-Drug Resistance (MDR) strains.The ESBL encoding genes were found in 21 isolates with blaSHV detected in 14(66.7%), blaTEM 3(14.3%), blaOXA-1 2(9.5%) blaCTX-M-9 and blaCTX-M-4 1(4.8% isolates each. 24 isolates were confirmed to phylogroups A, B1, B2, D with phylogroup B2 the most predominant 10(41.7%) then 7(29.2%) phylogroup D, 4(16.7%) B1 and 3(12.5%) A. This study has established “Suya” as potential reservoir of E. coli and the observable high degree of resistance of the isolates to antimicrobial agents gave clues to health practitioners on the best antibiotics of choice in treating E. coli infections.

Antimicrobial resistance, public health, “Suya”, Escherichia coli, Phylogenetic grouping.

"ANTIMICROBIAL RESISTANCE PROFILE AND PHYLOGENETIC GROUPING OF ESCHERICHIA COLI ISOLATED FROM “SUYA” SOLD IN NASARAWA STATE, NIGERIA.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.11, Issue 6, page no.b441-b457, June-2024, Available :http://www.jetir.org/papers/JETIR2406152.pdf

"ANTIMICROBIAL RESISTANCE PROFILE AND PHYLOGENETIC GROUPING OF ESCHERICHIA COLI ISOLATED FROM “SUYA” SOLD IN NASARAWA STATE, NIGERIA.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.11, Issue 6, page no. ppb441-b457, June-2024, Available at : http://www.jetir.org/papers/JETIR2406152.pdf