• ANJALI KUMARI
• Dr.Agam Gupta

Ocimum tenuiflorum (krishna tulsi) is most sacred plant of the India.It is cultivated for medicinal and religious purposes. It has many beneficial properties namely anti-oxidative, antimicrobial, antistress, antiviral and many others that’s why this plant is also given the term“Queen of Herbs”.Tulsi extract shows inhibitory effects against pathogens such as Klebsiella pneumoniae MTCC 109. The aim of this study was to assest the antimicrobial effects of ethanol and distilled water (aqueous) leaf extracts of O.tenuiflorum against pathogenic bacteria name K. pneumoniae MTCC 109 to determine their potentials as antibacterial agent. Leaves were separated from the stem then washed in clear water and dried (for 6 days) under the shady area and crushed in an Mortar and Pestle until a homogenous powder was obtained. Ethanolic and distilled water (aqueous) extract was prepared using soxhlet extraction method. The culture media was Muller Hinton Agar (MHA) Both extracts were serially diluted at quantities at the concentration of 1, 1/10, and 1/100 and for positive control antibiotic Gentamicin used. Disc diffusion method was done by soaking discs in these five various concentration and then placing on both cultured plates respectively.Both plates were left in BOD incubator for 24 hours. The activity of ethanol and distilled water Tulsi extract against K. pneumoniae MTCC 109 was found at a concentration of 55.25% and 50% respectively. The ethanol as well as distilled water(aqueous) both shows similar zone of inhibition against the bacteria Klebsiella pneumoniae MTCC 109. This study would be useful for the pharmacist for preparation of specific and more effective antimicrobial formulations by using Krishna tulsi species . in ancient medicinal traditions and suggest that some of the plant extracts possess compounds with antimicrobial properties that can be used as antimicrobial agents in new drugs for the therapy of infectious diseases caused by pathogens. It is safer to use as an herbal medicine as compare to chemically synthesized drug.

Ocimum tenuiflorum, Ethanol extract, Distilled water extract, Klebsiella pneumoniae MTCC 109, Disc Diffusion .

"ASSESSMENT OF ANTI-BACTERIAL ACTIVITY OF TULSI (O.TENUIFLORUM) AGAINST KLEBSIELLA PNEUMONIAE MTCC 109", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.11, Issue 6, page no.b865-b874, June-2024, Available :http://www.jetir.org/papers/JETIR2406195.pdf

"ASSESSMENT OF ANTI-BACTERIAL ACTIVITY OF TULSI (O.TENUIFLORUM) AGAINST KLEBSIELLA PNEUMONIAE MTCC 109", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.11, Issue 6, page no. ppb865-b874, June-2024, Available at : http://www.jetir.org/papers/JETIR2406195.pdf