• Ms. Pratiksha S. Hiwarale
• Mr Prashant S. Wake
• Mr. Shreyash S. Hiwarale

Abstract: In the face of escalating antimicrobial resistance, there is a pressing need for novel therapeutic approaches. Traditional herbal medicine presents a promising avenue for exploration due to its historical use and reported pharmacological properties. This study investigates the antimicrobial potential of a semi-solid formulation comprising ethanolic extracts from Vernonia cinerea, alongside other medicinal plants commonly used in Ayurvedic medicine, against a panel of pathogenic fungi and bacteria. Vernonia cinerea, also known as Sahadevi, has been traditionally utilized in treating various ailments, exhibiting diverse pharmacological activities including antibacterial effects. Ten herbs, collectively known as "Dashapushpam," were selected based on their traditional use and screened for antimicrobial activity. The formulation was prepared by combining ethanolic extracts of these plants and oils in varying ratios. The antimicrobial efficacy of the combinations was evaluated through standardized assays. Results revealed promising antimicrobial activity against a spectrum of pathogenic fungi and bacteria, suggesting the potential of the herbal semi-solid formulation as a natural alternative for combating microbial infections. This research underscores the importance of exploring traditional herbal remedies in the pursuit of novel antimicrobial agents, particularly amidst the current global challenge of antimicrobial resistance.

Index Terms-Vernonia cinerea Linn, Antimicrobial activity, Soxhlet extraction, Petroleum ether extraction, Ethanolic extract, Hydroalcoholic solution, Phytochemical screening, Thin Layer Chromatography (TLC), Rf value, Quercetin, Herbal cream formulation, Agar well diffusion method, E. coli, S. aureus, Compatibility study (FT-IR), Flavonoids, Extract yield, Skin permeation test, Stability study, Homogeneity, Viscosity, Spread ability.

"FORMULATION & EVALUATION OF ANTIMICROBIAL HERBAL CREAM CONTAINING EXTRACT OF LEAVES/PLANT GENIUS VERNONIA", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.11, Issue 6, page no.c841-c861, June-2024, Available :http://www.jetir.org/papers/JETIR2406300.pdf

"FORMULATION & EVALUATION OF ANTIMICROBIAL HERBAL CREAM CONTAINING EXTRACT OF LEAVES/PLANT GENIUS VERNONIA", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.11, Issue 6, page no. ppc841-c861, June-2024, Available at : http://www.jetir.org/papers/JETIR2406300.pdf