• Navaneetha Nambingari
• Markandeya Namani

Ruthenium (II) complexes with polypyridyl ligands have been extensively studied as promising functional molecules due to their unique photochemical and photophysical properties as well as cytotoxic properties. Herein ,an intercalative ligand, MTPIP [ MTPIP = [2-(4-methylthio)phenyl)-1H-imidazo [4,5-f][1,10] phenanthroline] and its mononuclear Ru(II) polypyridyl complex [Ru(A)2(MTPIP)]+2 (A=1,10–phenanthroline) have been synthesized and characterized by spectroscopic techniques such as elemental analysis,UV-Vis,IR,1H NMR,13C NMR and ESI-Mass. The interaction of the synthesized complex with DNA was studied using Biophysical Methods (Absorption, emission and viscosity methods).The study reveals that the Ru(II) polypyridyl complex bind to DNA preponderantly by intercalation. The complex showed absorption, hyperchromism in its UV-Vis spectrum with DNA. The binding constant Kb from UV-Vis absorption studies was 2.5x105 and Stern-Volmer quenching constant(Ksv) from fluorescence studies was 5.5x103 .Finally viscosity measurements revealed that the binding of the complex with CT-DNA is groove binding. In photo cleavage studies the complexes successfully cleaved the pBR 322 DNA. Ru(II) polypyridyl complex was examined for their antimicrobial activity. This was assessed against antibacterial (Staphylococcus, Bacillus, E.Coli & Klebsiella) and anti fungal (Candida & Aspergillus). The cytotoxic activity of these Ru(II) complex was carried by MTT assay with MCF-7 cell lines.MTT assay of the complex reveal good anticancer activity against MCF-7 cell line is IC50 =14.54±0.19 µg/ml indicating correlation with DNA binding affinity.

"Study of Ru (II) Polypyridyl Complex - Synthesis, Characterization, DNA-Binding, Antimicrobial activity and Cytotoxicity ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.11, Issue 6, page no.e332-e345, June-2024, Available :http://www.jetir.org/papers/JETIR2406445.pdf

"Study of Ru (II) Polypyridyl Complex - Synthesis, Characterization, DNA-Binding, Antimicrobial activity and Cytotoxicity ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.11, Issue 6, page no. ppe332-e345, June-2024, Available at : http://www.jetir.org/papers/JETIR2406445.pdf