• Deepthi Vankela
• Ramya G.N.V.S
• Nandini P
• Reddy O.V.S

The main output of the next generation sequencing is determination of sequence data of DNA or RNA in a vast amount. This sequencing is represented as a series of bases of nucleotides-Adenine, Thymine, Cytosine, Guanine for DNA and Adenine, Uracil, Guanine, Cytosine for RNA. This provides the genetic code of sample which is being sequenced. The first-generation sequencing such as Sanger sequencing analysis which is a time-consuming method when compared with second generation sequencing known as NGS. It may take time from few hours to some days but NGS complete the data sequence of DNA or RNA with in a single day (1). NGS also known as High-Throughput Sequencing (HTS), Massively Parallel Sequencing (MPS), Second generation sequencing etc. This review reflects the importance of NGS methodology and its applications in a simple manner. NGS, a revolutionary advancement over the first-generation Sanger sequencing, enables rapid and comprehensive sequencing of DNA and RNA. NGS deciphers the genetic code of samples with unprecedented speed and efficiency. This review underscores the significance of NGS technology and explores its wide-ranging applications, highlighting its transformative impact on genomic research and diagnostics.

NGS methodology, Applications, Advantages and Disadvantages.

"Comprehensive review on Next Generation Sequencing methodology and its applications ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.11, Issue 6, page no.g46-g54, June-2024, Available :http://www.jetir.org/papers/JETIR2406608.pdf

"Comprehensive review on Next Generation Sequencing methodology and its applications ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.11, Issue 6, page no. ppg46-g54, June-2024, Available at : http://www.jetir.org/papers/JETIR2406608.pdf