• G. KUMARESAN
• A.KUMARESAN

Liquid biofertilizer are special liquid formulation containing not only the desired micro organisms and their nutrients, but also special cell protectants or substances that encourage formation of resting spore or cyst for longer shelf life and tolerance to advanced condition. In this experiment, the authors evaluated different concentrations of four different chemical amendments viz, polyvinyl pyrollidone (PVP), glycerol, gum arabica, and trehalose for their ability to support growth and promote survival of Pseudomonas fluorescens in King’s B broth during the storage. Some concentrations of various additives to King’s B broth promoted higher Pseudomonas population compared to Pseudomonas cells in King’s B broth alone. Liquid Pseudomonas bioinoculant formulated with trehalose (10mM) promoted long-term survival of Pseudomonas followed by glycerol (10mM) gum arabica (0.3%) and PVP (2%) and they supported 108 cells/ml up to 11 months of storage under ambient temperature (28°C to 32°C), whereas control (talc powder carrier) recorded the same population upto 5 months only. The results indicated that the liquid formulation of Pseudomonas could be used more effectively than the carrier based formulation.

Bioinoculants, Pseudomonas fluorescens, King’s B broth, trehalose, liquid formulation, etc.

"DEVELOPMENT OF LIQUID FORMULATION AMENDED WITH DIFFERENT CHEMICAL ADDITIVES ON THE SURVIVAL OF PSEUDOMONAS FLUORESCENS ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 5, page no.443-451, May-2019, Available :http://www.jetir.org/papers/JETIRBP06078.pdf

"DEVELOPMENT OF LIQUID FORMULATION AMENDED WITH DIFFERENT CHEMICAL ADDITIVES ON THE SURVIVAL OF PSEUDOMONAS FLUORESCENS ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 5, page no. pp443-451, May-2019, Available at : http://www.jetir.org/papers/JETIRBP06078.pdf