• Firdous Bagban
• Shweta Patel

Our aim of the research is to isolate the Lactobacillus acidophilus bacteria from Yakult which is a dairy product and the application of the organism in probiotic ice-cream. The duration of the research is 4-5 months. For the research, around 20-25 research papers were referred. Probiotics can be defined as live microorganisms (bacteria or yeast) that can bring health benefits to humans or animal bodies, usually the maintenance and the improvement of the microbial balance of the intestinal environment. Ice cream is a dairy product with good potential to act as a food carrier for probiotic bacteria. The growing interest of consumers in therapeutic cultures into Ice cream to result in dietetic Ice cream. Lactobacilli are gram positive and catalase negative bacteria. Most of the dairy industry use Lactic acid bacteria as a starter culture for the manufacturing of fermented products like yoghurt, cheese and Yakut, etc. The purpose of our research is to manufacture a probiotic ice cream by inoculating Lactobacillus acidophilus in the ice cream and then to check the colony forming unit per ml on MRS medium and check the viability of the bacteria after frozen storage and to compare the prepared ice cream with the commercial ice cream. Among the most used organisms are those belong to the general of Lactobacillus and Bifidobacterium which are believed to have beneficial effects on human health, some of the benefits of probiotic foods are promotion of growth and digestion, setting effect on bowel movement, suppression of cancer, catering to lactose intolerance and lowering blood cholesterol levels, etc

Lactobacillus acidophilus, Probiotics, ice cream, health benefits, Rogosa medium

"Isolation of Lactobacillus acidophilus from Yakult and its application in Probiotic Ice-cream", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.10, Issue 6, page no.65-71, June-2023, Available :http://www.jetir.org/papers/JETIRFY06009.pdf

"Isolation of Lactobacillus acidophilus from Yakult and its application in Probiotic Ice-cream", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.10, Issue 6, page no. pp65-71, June-2023, Available at : http://www.jetir.org/papers/JETIRFY06009.pdf